H9c2 microarray
WebJul 25, 2024 · Arraystar lncRNA and mRNA array data for H9c2 myocytes from Hypoxia/reoxygenation model. Ischemia reperfusion (I/R) promotes the severity of … WebHypoxia suppresses proliferation and migration of H9c2 cells. (A) H9c2 cells were incubated in a hypoxic incubator under 1% O 2, 5% CO 2, and 94% N 2. Cell proliferation was determined via the CCK-8 assay for 72h and shown as the fold increase compared to 0 h. (B) Confluent H9c2 cells were scratched to generate wounds.
H9c2 microarray
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WebJul 16, 2024 · H9C2 cells (ProCell, Wuhan, China) were subcloned from BD1X rat embryonic heart tissue and cultured in Dulbecco’s modified Eagle medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin double-antibody. The culture environment was 37 ℃ in an incubator with 5% CO2and normal ambient oxygen levels. WebSplit sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding 1-3x10,000 cells/cm 2 using 0.25% trypsin or trypsin/EDTA; 5% CO 2; 37°C. Important to passage at sub-confluence, reclone periodically to keep differentiated characteristics. Cells will fuse to form myotubes if allowed to reach confluency.
WebMay 1, 2008 · H9c2 cells and metabolic inhibition. We purchased H9c2 cardiomyoblasts from American Type Culture Collection and cultured them in Dulbecco's modified Eagle's medium ... Microarray analysis reveals pivotal divergent mRNA expression profiles early in the development of either compensated ventricular hypertrophy or heart failure. WebAug 16, 2024 · H9C2 cells were used to generate a hypoxia/reoxygenation (H/R) in vitro model for the present study. Reverse transcription‑quantitative PCR and western blotting …
WebSep 30, 2024 · To evaluate whether EHT 1864, a Rac1 inhibitor, could affect cell viability, we pretreated H9c2 cells with EHT 1864 (20 μ M) for 30 min and then treated them with glucose (8 and 32 mM) under normal and hypoxic conditions. As shown in Figure 1 (a), EHT 1864 significantly lowered cell viability by about 30%. WebJan 5, 2024 · What is the recommended seeding density for H9C2 cells (specifically in a plastic tc t25 flask)? We have been seeding at the recommended 10x10^4/cm^2 (250,000 in t25) and they are reaching …
WebH9c2 cells are an undifferentiated myoblasts cell line, and the immortalized nature of H9c2 cells with an active cell cycle is different from fully differentiated beating cardiomyocytes in vivo.
Web4th Apr, 2013. Eric R Gross. Depends on what your research focus is. Remember the hl1 cells are derived from the mouse atria. while although derived from myoblasts, h9c2 cells are supposed to be ... foiled chocolate heartsWebJan 28, 2024 · IH model of H9c2 cells was established as described previously . Briefly, H9c2 cells were transferred to hypoxic chamber with 1% O 2 at 37 °C for 35 min; then, … eft session notesWebH9c2 cells were plated in 24-well plates and cultured to 80–90% confluence for transient transfection with the miR-92a inhibitor (50 nM) or NC (50 nM), respectively. Immunocytofluorescence... foiled chocolate hearts wholesaleWebThis is key for this H9C2. Since your one is fresh from ATCC, you no need to worry much. All will be great. Later after you might notice that you need to do clone selection when you see cell... foiled crossword clue 8WebMicroRNA-140-5p aggravates doxorubicin-induced cardiotoxicity by promoting myocardial oxidative stress via targeting Nrf2 and Sirt2. Clinical application of doxorubicin (DOX), … foiled chocolate bottles with liquor fillingWebH9c2 (2-1) CRL-1446 ™. H9c2 (2-1) is a subclone of the original clonal cell line derived from embryonic BD1X rat heart tissue that exhibits many of the properties of skeletal muscle. … eftsetup fleetcoremail.comWebChronic cardiac pressure overload, caused by conditions, such as hypertension, induces pathological hypertrophic growth of myocardium and vascular rarefaction, with largely unknown mechanisms. Here, we described that expression of the PKR2 eft settings explained