http://www.iotword.com/3663.html WebMar 6, 2024 · 下面对几个常用的选项详细解释一下:. -F # 指定区间注释文件的格式,默认是GTF. -o # 输出文件:可输出raw counts的txt文本及raw counts的summary文本. -p # 针对paired-end数据. -g # 指定统计的meta-feature名称,默认是gene_id. -t # 指定统计的feature名称, 默认是exon。. 可有 CDS,exon ...
featureCounts 软件说明 - 庐州月光 - 博客园
WebApr 28, 2024 · 转录组定量工具-featureCounts安装及使用. 计算表达量可以用 StringTie、Htseq-count或featureCount ,第一次做转录组分析时,参照了一篇Cell的子刊文章的分析方法,里面使用的STAR+featureCount,就直接用了这个软件,也就没再使用别的,回头看第一次使用时,发现好多细节 ... WebSep 16, 2024 · featureCounts软件使用与参考基因组比对后的bam文件计算比对到各个基因的read counts数。 注:可使用featureCounts -h 查看featureCounts软件的其他参数。 4. 运行结果. featureCounts运行完成后主要生产2个文件:*.txt和*.txt.summary。 *.txt.summary是对reads的统计结果,文件内容如下: miele cleaning tabs for dishwasher
featurecounts · GitHub Topics · GitHub
WebMay 23, 2024 · Question: Featurecounts for paired end RNA-seq reads not generating counts. I have paired end RNA-Seq reads that I aligned to my reference genome using HISAT2 (alignment mostly >90%) and obtained BAM files, as a result. When I tried to obtain count information for these BAM files using the annotated file of the same build of the … Web1. 什么时候需要在feature级别计数? 2. 是否要计多重比对? 3. 是否该只用最优比对? When --primary is specified, the -M option will be ignored, meaning that a primary alignment will always be counted no matter the read is multi-mapped or not. 4. 是否要忽略重复reads? 5. pair-end模式下是否要用fragment ... WebfeatureCounts - a highly efficient and accurate read summarization program SYNOPSIS featureCounts [options] -a -o input_file1 [input_file2] ... newton wallacetown church service