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WebApr 25, 2024 · The bifunctional enzyme β-carotene hydroxylase (CrtZ) catalyzes the hydroxylation of carotenoid β-ionone rings at the 3, 3’ position regardless of the presence of keto group at 4, 4’ position, which is an important step in the synthesis of astaxanthin. The level and substrate preference of CrtZ may have great effect on the amount of … WebSep 1, 2024 · This study engineered β-carotene ketolase CrtW and β-carotene hydroxylase CrtZ to improve biosynthesis of astaxanthin in Escherichia coli. Firstly, crtW was … WebSep 1, 2024 · This study engineered β-carotene ketolase CrtW and β-carotene hydroxylase CrtZ to improve biosynthesis of astaxanthin in Escherichia coli.Firstly, crtW was randomly mutated to increase CrtW activities on conversion from β-carotene to astaxanthin.A crtW* mutant with A6T, T105A and L239M mutations has improved 5.35 … اصلاح صورت با بند انداز برقی نی نی سایت