Crotonyl-coa carboxylase/reductase
WebFeb 6, 2015 · Herein, we report the rational control of polyketide extender units by the structure-based engineering of a crotonyl-CoA carboxylase/reductase (CCR), in the … WebThe use of methylmalonyl-CoA epimerase (MCEE) to improve stereoselectivity in crotonase-mediated biocatalysis is exemplified by the …
Crotonyl-coa carboxylase/reductase
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WebNov 2, 2015 · These findings deepen our understanding of the molecular mechanisms of the CCRs, which will serve as versatile biocatalysts for the manipulation of building blocks, and set the stage for the rational design of polyketide biosynthesis. Keywords: biosynthesis; crotonyl-CoA carboxylase/reductase; polyketides; protein engineering. WebMay 9, 2024 · One of the most efficient classes of carboxylating enzymes are enoyl-CoA carboxylases/reductases (Ecrs), which outcompete the plant enzyme …
WebJul 28, 2009 · To demonstrate the function of SalG as a 4-chlorocrotonyl-CoA carboxylase/reductase and establish the authenticity of chloroethylmalonyl-CoA as an intermediate, we biochemically characterized recombinant SalG, which was expressed as an octahistidyl-tagged protein in Escherichia coli. Webcomplexes, crotonyl-CoA carboxylase/reductase AntE in complex with NADP+ (PDB: 4Y0K) and 2-octenoyl-CoA Figure 1. Reaction scheme and structural organization of the K. setae ECR complex. (a) Carboxylation reaction scheme of ECR. (b) Anisotropic B-factors of the tetramer of the different ECR complexes
WebBerg et al. (Reports, 14 December 2007, p. 1782) reported the discovery of an autotrophic carbon dioxide–fixation pathway in Archaea and implicated a substantial role of this pathway in global carbon cycling based on sequence analysis of Global ... WebSep 10, 2015 · Extender unit engineering: The crotonyl-CoA carboxylase/reductase AntE was engineered to catalyze the …
WebMay 12, 2024 · Poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate] [P(3HB-co-3HHx)] is a practical kind of bacterial polyhydroxyalkanoates (PHAs). A previous study has established an artificial pathway for the biosynthesis of P(3HB-co-3HHx) from structurally unrelated sugars in Ralstonia eutropha, in which crotonyl-CoA carboxylase/reductase (Ccr) and …
WebNov 17, 2013 · A crotonyl-CoA reductase-carboxylase independent pathway for assembly of unusual alkylmalonyl-CoA polyketide synthase extender units Lauren Ray Timothy R. Valentic the oaks of acworthWebA crotonyl-CoA carboxylase/reductase independent pathway for unusual extender unit biosynthesis Nature Communications Dec 2016 Type I … the oaks of brier creekWebFirst, the C 4 compound crotonyl-CoA (a condensation product of two acetyl-CoA molecules) is carboxylated into the C 5 intermediate (2S)-ethylmalonyl-CoA. This reaction is catalyzed by crotonyl-CoA carboxylase/reductase (CCR), which represents a novel class of biotin-independent reductive acyl-CoA carboxylases that use NADPH as a reductant . the oaks oakworth road keighleyWebFeb 19, 2024 · Butyrogenesis proceeds through butyryl-coenzyme A (butyryl-CoA) generation from acetoacetyl-CoA via the intermediates β-hydroxybutyryl-CoA and crotonyl-CoA. Butyryl-CoA is then converted to butyrate via two pathways, one of which involves the generation of butyrate-phosphate via ptb , which is further converted to butyrate via buk [ … the oaks nursing home new bedford maWebJun 2, 2009 · Amino acid sequence analysis showed that crotonyl-CoA carboxylase/reductase is a member of the medium-chain dehydrogenase/reductase … the oaks nursing home warner robins gaWebThis review covers the emerging biosynthetic role of crotonyl-CoA carboxylase /reductase (CCR) homologs in extending the structural and functional diversity of polyketide natural products. CCRs catalyze … the oaks nursing home scWebIn this study, a TetR-type activator, CcrR, was shown to regulate the expression of crotonyl-CoA reductase/carboxylase, an enzyme of the ethylmalonyl-CoA pathway involved in the assimilation of C(1) and C(2) compounds in Methylobacterium extorquens AM1. A ccrR null mutant strain was impaired in its ability to grow on C(1) and C(2) compounds ... the oaks of arlington apartments